ZOOEMBRIO · ART media · equine & bovine
Russian manufacturer for the full media cycle — washing, rinsing, vitrification and warming. Local supply, application protocols and documentation with every lot.
Equine line in serial production; cattle ART media launching under the same quality standard.
In-house manufacturing in Russia — predictable lead times without import uncertainty.
Application protocols, lot certificates, MEA and endotoxin control, ISO sterile production.
Whether you work with mares, cows, or both — protocols, paperwork and supply from one Russian source.
The ZOOEMBRIO protocol is built around four sequential stages. Each medium addresses a specific task at a defined point in the process.
Equine breeding increasingly relies on technologies that preserve and pass on genetics from valuable mares without the classical path of breeding, pregnancy and embryo flushing. The in vivo protocol with transvaginal oocyte aspiration (OPU) provides direct access to gametes from the ovary of a live mare — without requiring her to be bred and carry an embryo.
For breeding farms, reproductive centers and owners of performance mares, this opens practical options: collect oocytes from outstanding donors in a convenient seasonal window without interrupting competition careers or overloading the uterus with repeat pregnancies. Cells can be vitrified and stored in liquid nitrogen, while fertilization and transfer are scheduled around recipient mares and laboratory capacity. Success of the entire program starts at the first laboratory step — high-quality washing in a specialized medium. The ZOOEMBRIO line covers the full post-aspiration cycle: from washing and rinsing to vitrification and devitrification.
Oocytes are collected from champions and leading broodmares without the burden of carrying offspring. The donor can keep competing, attend selection events or remain available on a limited schedule while her genetic material stays accessible for the breeding program.
Aspiration is scheduled between events or outside the competition season. The procedure takes 30–40 minutes under sedation; the mare can return to training afterward. For sport horse producers, this is a way to combine athletic careers with breeding work.
When conventional reproduction fails — chronic endometritis, age-related changes, reproductive tract injuries, low fertilization rates — direct oocyte collection from the ovary opens a path to continue the line without giving up valuable genetics.
Aspiration is performed when follicles of suitable size are present, without strict dependence on a single ovulation day. Repeat procedures are possible at 2–3 week intervals. A breeding farm can distribute laboratory and recipient workload across the full season.
Oocytes and embryos are vitrified and stored in liquid nitrogen for transfer at a convenient time — including another region or the next season. This removes the need for tight synchronization between donor and recipient mares and simplifies logistics for large breeding programs.
After aspiration, oocytes are highly sensitive to medium composition, temperature and handling time. Proper washing in TSM-Asp preserves cell morphology and sets the success of the entire subsequent protocol — rinsing, vitrification and devitrification. Media for each stage must therefore work as a coordinated system.
The diagram shows the four protocol stages and ZOOEMBRIO media for each step. A detailed description of every stage is on the Technology page.
Learn more about the technologyFour product groups cover the complete equine in vivo protocol.
Medium for washing oocytes during aspiration and primary follicular fluid processing.
Medium for rinsing cells between protocol stages and preparing for cryopreservation.
Media kit for vitrification of equine embryos and oocytes.
Media kit for devitrification of vitrified embryos and oocytes.
What changed in the lab or on the farm after switching to ZOOEMBRIO media.
OPU days twice a week
Before the switch they built TSM volume from multiple vials — long OPU days meant extra openings and sterility risk. With 1000/3000 ml bags the line connects once; Wash and ViT are ordered as one pack against the mare schedule. Over the breeding season they ran forty-plus aspirations on a single supplier stack, without bridging lots from imports.
Field schedule + media stock
The pain point was logistics more than formula: an imported kit slipped at peak season. Now Wash and TSM-Asp go into the order 10–14 days before a procedure block; lot certificates go straight into the farm vet archive. The embryologist notes Wash clarity stays consistent on the warming stage across lots.
Vitrification & embryo bank
They did not rewrite the full SOP for a foreign kit: ViT1/ViT2 and three-step WaM slotted into existing timings. Two seasons with no downtime from missing media; temperature/exposure questions were closed in one call with a ZOOEMBRIO technologist and written into the internal checklist.
Not ad slogans — what embryologists and vets say after changing supplier, with protocol detail.
«On a day with four mares back-to-back, one bag versus five vials is not “nice to have” — it is fewer points of failure. TSM-Asp behaves the same from March to September; Wash viscosity does not drift lot to lot. We finally stopped keeping an imported kit “just in case”.»
«As farm vet I need three things on the paperwork: expiry, lot certificate and arrival date. With ZOOEMBRIO that is predictable. Once a truck slipped a day — they warned us and we moved the OPU block. With imports there was no dialogue: either the distributor had stock or “wait for the container”.»
«ViT2 sits at the step where colleagues used to complain about “different drag” from foreign media. Here the drop feels familiar lot to lot — you see it under the stereomicroscope. WaM1→3 did not force new dishes or timers; we keep the ZOOEMBRIO protocol next to our laminated checklist.»
«We are looking at cattle because equine already works: we do not want another single foreign line for cows. For now we take a consult and a test volume under our OPU SOP — same document language as for horses matters.»
ZOOEMBRIO manufactures ART media under sterile conditions at our own facility. ISO-certified production — we control formulation, preparation and filling from raw materials to finished batch.
Media produced under controlled sterile conditions to ISO standards.
Formulas developed and tested — MEA and endotoxin testing passed.
Release testing for every batch, regular MEA and endotoxin monitoring.
ZOOEMBRIO is a Russian manufacturer of ready-to-use ART media for horses and cattle: washing, rinsing, vitrification and warming.
Domestic sterile manufacturing in Russia, application protocols, lot certificates and predictable shipping without import lead times.
No. ZOOEMBRIO (zooembrio.ru) is a Russian ART media manufacturer. Zebuembryo is a different organisation. Human IVF clinics named «Эмбрио» are unrelated.
Send a request — we will share equine and/or cattle pricing, protocols and lot documentation.
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